Functions of the Duplicated Hik 31 Operons in Central 1 Metabolism and Responses to Light , Dark and Carbon 2 Sources in Synechocystis Sp . Strain Pcc 6803
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چکیده
FUNCTIONS OF THE DUPLICATED HIK31 OPERONS IN CENTRAL 1 METABOLISM AND RESPONSES TO LIGHT, DARK AND CARBON 2 SOURCES IN SYNECHOCYSTIS SP. STRAIN PCC 6803. 3 4 Sowmya Nagarajan, Debra M. Sherman , Isaac Shaw 3 and 5 Louis A. Sherman * 6 Department of Biological Sciences 1 and Life Sciences Microscopy facility, 7 Department of Horticulture , Purdue University, West Lafayette, Indiana 47907 8 9 Running Title: Non-redundant functions for duplicated Hik31 operons 10 11 *Corresponding Author: 12 Prof. Louis Sherman 13 Purdue University 14 Dept. of Biological Sciences 15 915 W. State. St. 16 West Lafayette, IN 47907 17 Phone: 765-494-8106 18 Fax: 765-494-0876 19 [email protected] 20 21 3 Current address: Stritch School of Medicine, Loyola University, Maywood, 22 Illinois 60153. 23 24 Copyright © 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.06207-11 JB Accepts, published online ahead of print on 11 November 2011
منابع مشابه
Functions of the duplicated hik31 operons in central metabolism and responses to light, dark, and carbon sources in Synechocystis sp. strain PCC 6803.
There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to examine whether the duplicated copies have the same or different functions and gene...
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The study of the primary metabolism of cyanobacteria in response to light conditions is important for environmental biology because cyanobacteria are widely distributed among various ecological niches. Cyanobacteria uniquely possess circadian rhythms, with central oscillators consisting from three proteins, KaiA, KaiB, and KaiC. The two-component histidine kinase SasA/Hik8 and response regulato...
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The cyanobacterium Synechocystis sp. strain PCC 6803 exhibited circadian rhythms in complete darkness. To monitor a circadian rhythm of the Synechocystis cells in darkness, we introduced a PdnaK1::luxAB gene fusion (S. Aoki, T. Kondo, and M. Ishiura, J. Bacteriol. 177:5606-5611, 1995), which was composed of a promoter region of the Synechocystis dnaK1 gene and a promoterless bacterial luciferas...
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The plasmid hik31 operon (P3, slr6039-slr6041) is located on the pSYSX plasmid in Synechocystis sp. PCC 6803. A P3 mutant (ΔP3) had a growth defect in the dark and a pigment defect that was worsened by the addition of glucose. The glucose defect was from incomplete metabolism of the substrate, was pH dependent, and completely overcome by the addition of bicarbonate. Addition of organic carbon a...
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Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 are model cyanobacteria from which the metabolism and adaptive responses of other cyanobacteria are inferred. Using stranded and 5' enriched libraries, we measured the gene expression response of cells transferred from reference conditions to stress conditions of decreased inorganic carbon, increased salinity, increased pH, and decreased...
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تاریخ انتشار 2011